Journal of Orofacial Sciences

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 11  |  Issue : 2  |  Page : 79--83

Effects of Vitamin D in Alveolar Bone Remodeling on Osteoblast Numbers and Bone Alkaline Phosphatase Expression in Pregnant Rats During Orthodontic Tooth Movement


Puteri Nazirah B Megat Badarul Hisham, Ida B Narmada, Alida Alida, Dwi Rahmawati, Alexander P Nugraha, Nurul A. R Putranti 
 Orthodontic Department, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia

Correspondence Address:
Ida B Narmada
Jl. Mayjen. Prof. Dr. Moestopo 47, Surabaya
Indonesia

Introduction: The vitamin D effect on orthodontic tooth movement in pregnant women remains unknown. The aim of this study was to investigate the post administration of vitamin D effect on osteoblast numbers and bone alkaline phosphatase (BALP) expression in the tension side in pregnant rats during orthodontic tooth movement. Materials and Methods: This study was an in vivo animal experiment; 28 healthy female Wistar rats (Rattus norvegicus) (16–20 weeks’ old) were divided into four groups, with or without intramuscular administration of vitamin D, which were to be observed after 7 and 14 days. Pregnancy was induced with pregnant mare serum gonadotropin and human chorionic gonadotropin. Nickel–titanium coil springs with 30 g/mm2 of force were connected between the right maxillary incisors and the right maxillary first molar. After 7 and 14 days, the animals were sacrificed. Statistical Analysis Used: Analysis of variance with post hoc test (P < 0.05) was performed based on the results of a Levene’s test and a Kolmogorov–Smirnov test (P > 0.05). Results: The highest number of osteoblasts occurred in the C-7 group with mean ± standard deviation of 20.54 ± 8.4. Statistically significant differences were seen in decreased osteoblast number between groups (P = 0.001, P < 0.05). The highest BALP expression was in the E-7 group (3.40 ± 1.625). Nevertheless, no statistically significant difference was observed between the groups (P = 0.240, P > 0.05) in the expression of BALP. Conclusion: The post administration of vitamin D during orthodontic tooth movement in pregnant rats produced no significant enhancement on BALP expression and osteoblast number.


How to cite this article:
Megat Badarul Hisham PB, Narmada IB, Alida A, Rahmawati D, Nugraha AP, Putranti NA. Effects of Vitamin D in Alveolar Bone Remodeling on Osteoblast Numbers and Bone Alkaline Phosphatase Expression in Pregnant Rats During Orthodontic Tooth Movement.J Orofac Sci 2019;11:79-83


How to cite this URL:
Megat Badarul Hisham PB, Narmada IB, Alida A, Rahmawati D, Nugraha AP, Putranti NA. Effects of Vitamin D in Alveolar Bone Remodeling on Osteoblast Numbers and Bone Alkaline Phosphatase Expression in Pregnant Rats During Orthodontic Tooth Movement. J Orofac Sci [serial online] 2019 [cited 2020 May 30 ];11:79-83
Available from: http://www.jofs.in/article.asp?issn=0975-8844;year=2019;volume=11;issue=2;spage=79;epage=83;aulast=Megat;type=0