ORIGINAL ARTICLE
Year : 2012  |  Volume : 4  |  Issue : 1  |  Page : 37-42

Liquid dish washing soap: An excellent substitute for xylene and alcohol in hematoxylin and eosin staining procedure


1 Department of Oral Pathology, Navodaya Dental College, Raichur, Karnataka, India
2 Department of Periodontology, Navodaya Dental College, Raichur, Karnataka, India
3 Department of Oral Medicine and Radiology, Navodaya Dental College, Raichur, Karnataka, India
4 Department of Community Medicine, Navodaya Dental College, Raichur, Karnataka, India

Correspondence Address:
Surekha Ramulu
Department of Oral Pathology, Navodaya Dental College, Raichur, Karnataka
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0975-8844.99890

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Aims: Liquid dish washing solution (DWS) was used as a substitute for xylene to dewax tissue sections during hematoxylin and eosin (H and E) staining. The aim was to test and compare the hypothesis that xylene-ethanol free (XEF) sections deparaffinized with diluted DWS are better than or at par with the conventional H and E sections. Materials and Methods: Fifty paraffin-embedded tissue blocks was included. One section was stained with conventional HandE (group A) and the other with XEF HandE (group B) staining method. Slides were scored for parameters: nuclear, cytoplasmic, clarity, uniformity, and crispness of staining. Z test was used for statistical analysis. For accuracy of diagnosis, sensitivity, specificity, positive predictive value, and negative predictive value were tested. Results: Adequate nuclear staining was noted in 94% in group A and 96% in group B, ­adequate cytoplasmic staining in 92% in group A and 86% in group B, clarity in 94% of group A and 96% of group B sections, uniform staining in 92% of group A and 80% of group B sections, crisp stain in 96% of group A and 88% of group B sections, and 94% of group A sections stained adequately for diagnosis as compared with 90% in group B sections. Conclusion: Liquid DWS can be used as an alternative and effective substitute to xylene and ethanol in routine HandE staining procedure.


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